Loading Rates of Dust and Bioburden in Dwellings in an Inland City of Southern Europe

Sampling campaigns indoors have shown that occupants exposed to contaminated air generally exhibit diverse health outcomes. This study intends assess the deposition rates of total settleable dust and bioburden in indoor dwellings onto quartz fiber filters electrostatic collectors (EDCs), respectively. EDC extracts were inoculated malt extract agar (MEA) dichloran glycerol (DG18) agar-based media used for fungal contamination characterization, while tryptic soy (TSA) was applied bacteria assessment, violet red bile (VRBA) Gram-negative bacteria. Azole-resistance screening molecular detection by qPCR also performed. Dust loading ranged from 0.111 3.52, averaging 0.675 μg cm−2 day−1. Bacterial counts undetectable 16.3 colony-forming units (CFU) m−2 day−1 2.95 CFU TSA VRBA, Fungal 1.97 35.4 MEA, 48.8 DG18. Penicillium sp. presented highest prevalence MEA (36.2%) Cladosporium DG18 (39.2%). It possible observe: (a) loadings higher with pets; (b) species considered indicators harmful contamination; (c) Aspergillus section Candidi identified supplemented voriconazole posaconazole; (d) specific housing typologies (e) characteristics influencing microbial contamination.